BIOCHEMICAL AND KINETIC PROPERTIES OF CRUDE PHOSPHOLIPASE A2 FROM NAJA NIGRICOLLIS VENOM
Snake envenomation has been a source of concern worldwide due to potential mortality risks associated with it. This study was carried out to investigate the biochemical and kinetic properties of crude phospholipase A2 (PLA2) from Naja nigricollis venom. Biochemical and kinetic properties of the venom was determined using standard procedure. Though the results showed that the enzyme PLA2 is stable at pH 4 and 6, the optimum pH of activity of the PLA2 is 3, while the optimum temperature of activity of the enzyme is 70 oC. Of all the activators used (CoCl2, CaCl2, MnCl2 and MgCl2), MgCl2 was the highest activator of the enzyme activity at 5 mM. The suggestive amino acids in the active site of the PLA2 were aspartate and glutamate. The kinetic parameters, Km and Vmax of the PLA2 were found to be 0.6 mg/ml and 3.3 μmol/min respectively. These findings could lend support to more understanding of biochemical and kinetic properties of crude Naja nigricollis PLA2.