PREVALENCE OF EXTENDED SPECTRUM BETA-LACTAMASE PRODUCING KLEBSIELLA PNEUMONIAE AND PSEUDOMONAS AERUGINOSA AMONG WOMEN WITH URINARY TRACT INFECTIONS ATTENDING ANTENATAL CARE IN KADUNA, NIGERIA
This study determined the prevalence of Extended Spectrum B-lactamase (ESBL) and their resistance gene producing Klebsiella pneumoniae and Pseudomonas aeruginosa among women with urinary tract infections attending antenatal care in Barau Dikko Teaching Hospital Kaduna, Nigeria. A total of 230 mid-stream urine samples were identified using cultural (MacConkey agar and Cetrimide agar respectively) and biochemical methods. Isolates were further screened for Extended Spectrum Beta-lactamase (ESBLs) production using double disc synergy test (DDST). Positive ESBLs isolates were further subjected to multiplex PCR for resistance gene determination. The overall UTIs prevalence was 22.17% (51/230) among which 14.78% (34/230) were Klebsiella pneumonia and 7.39% (17/230) were Pseudomonas aeruginosa. The overall prevalence of Extended spectrum β-lactamase was 0.87% (2/230) among which 11.76% (2/17) were Klebsiella pneumoniae and 14.29% (2/14) were Pseudomonas aeruginosa. Extended spectrum Beta-lactamase genes were detected only in Klebsiella pneumonae and not in Pseudomonas aeruginosa. The resistance genes identified in ESBLs producing Klebsiella pneumonae were blaSHV and blaCTX-M, both in equal proportion; blaTEM was not detected. Antimicrobial susceptibility testing and ESBL production monitoring are therefore recommended in patients.